The Most Trusted
          Name in DNA Testing

Cell Line Authentication

The legitimacy of research findings produced from biomedical research performed with cultured cells can become threatened by contamination and misidentification. For this reason, the National Institutes of Health (NIH) has recommended and issued guidelines for STR cell line authentication, in order for researchers to receive grants and/or to be published. (click here to view the NIH guidelines)

DDC, in partnership with Promega Corporation, is proud to provide fast, accurate cell line authentication.

Who Can Benefit

• Biomedical Researchers
• University Research Programs

What We Need

• Purified DNA - 10-20 μl at a minimum of 1 ng/μl
     - or -
• Cell pellet containing 1x10⁵ cells

What You Receive

• STR Allele Report and an Explanation of Results
• Electropherogram
• Fast Turnaround Time-Results in 5 days

StemElite™ ID System & PowerPlex® 16

StemElite™ ID and/or PowerPlex® 16 provide powerful and complete systems for STR cell line authentication. The StemElite™ ID system incorporates a sensitive marker that specifically detects the presence of contaminating mouse DNA. Both StemElite™ ID and PowerPlex® 16 use all the standard American Type Culture Collection (ATCC) markers for authenticating cell lines.

DDC's Testing Offers

• Unparalleled quality with 15 years of experience in Human Identification using STR Profiling
• Knowledgeable, accessible customer service
• Domestic and international laboratory accreditations from AABB, CLIA, ACLASS for ISO/IEC 17025, CAP, and NYSDOH

For more information please call 1-800-929-0815. To learn more about the science behind the testing process please visit our partner at www.promega.com.

DNA Testing Costs

StemElite™ ID............................ $149 per sample

PowerPlex® 16  ............................ $149 per sample

DNA Isolation  ............................ $25 per sample - Using Promega Wizard® SV Genomic Extraction

or call 1-800-929-0815 to Order Now!

Sample Shipping Guidelines

1. If sending cells, prepare a cell pellet containing at least 1 x 10⁵ cells.
2. Wash the centrifuged cell pellet with phosphate buffered saline (PBS) at least three times and place the cell pellet in a 1 ml vial or tube.
3. Ship the cell pellet on blue ice packs or dry ice.
4. If performing your own DNA isolation, please send 10 - 20 μl of purified DNA (at a minimum concentration of 1 ng/μl). We recommend using TE^-4 buffer (10mM Tris-HCl, 0.1mM EDTA [pH 8.0]). Please provide the concentration of DNA if known.
5. DNA samples in solution should be shipped on blue ice packs or dry ice. Dried DNA samples can be shipped at room temperature.
6. Fill out the shipment form and send the samples to our laboratory via overnight courier.
   Shipping Address:
      DNA Diagnostics Center
      Attn: Cell Line ID
      One DDC Way
      Fairfield, OH 45014

Ordering Process

To order and setup the Cell Line Authentication process follow these simple steps:

1. Download a PDF of the order form here or call DDC at 1-800-929-0815 to get started.
2. Complete Order Form and fax to 1-800-363-1707
3. Carefully follow shipping guidelines above, and send samples to:
      DNA Diagnostics Center
      Attention: Cell Line ID
      One DDC Way
      Fairfield, OH 45014
4. You will be notified upon sample arrival and receive a due date
5. Once the testing is completed, your results will be sent via your preferred method(s)

Click here to download ordering process PDF