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Human Cell Line Authentication and Mycoplasma Detection
DNA Diagnostics Center (DDC) provides fast, reliable, STR-based cell line authentication services to verify cell lines used in research. DDC's testing complies with the requirements of the American Type Culture Collection (ATCC) and other leading scientific organizations. We currently offer:
- Human Cell Line and Primary Cell Authentication
- Mycoplasma detection
As the world's largest DNA testing laboratory, DDC provides cell line authentication services to major corporations and research institutions, as well as to individual research projects. DDC offers:
- Unparalleled quality with 17 years of experience in Human Identification using STR Profiling.
- Worldwide laboratory accreditations from AABB, CLIA, ACLASS for ISO/IEC 17025, CAP, and NYSDOH.
- Knowledgeable, accessible customer service and large staff of Phd's.
- Over half a million STR tests performed each year.
The Need for Cell Line Authentication
In late 2007, an open letter by a group of scientists led by Dr. Roland Nardone spurred the National Institutes of Health (NIH) to recommend authenticating cell lines in scientific studies.
In early 2012, the ATCC issued its standard to address the problem of cell line misidentification and "delineate a standardized, universally applicable method for authenticating new and established human cell lines and human primary tissue used in research." This standard has been submitted for review and approval as an American National Standard by the American National Standards Institute.
Cell lines are often used in biomedical research to investigate disease pathways and therapies. However, recent reports estimate that about 20% of cell lines in the literature within the past 30 years are misidentified or contaminated, compromising not only the results of those experiments, but also pushing back general scientific progress as subsequent studies were designed based on erroneous data.
As a result, many funding agencies and peer-reviewed journals are now making cell authentication a requirement for consideration of proposals and submitted articles. While previous methods to authenticate cell lines were cost-prohibitive and time consuming, recent advances in DNA technology now allow for an affordable, quick method to authenticate human cell lines.
References
Below are recent articles discussing the development of cell line authentication guidelines.
General Recommendations for Authenticating Cells
• When a new line is first established or received.
• As a control measure at the start of a new study and concluding a study.
• At least once every 6 months in active culture.
• Before freezing down for storage.
• When unexpected results occur.
 Human Cell Line and Primary Cell Authentication
DDC offers the most comprehensive testing available by routinely using the PowerPlex®16, a panel of 15 STR markers (vs. the 8 recommended by the ATCC) and the amelogenin sex identification marker, to generate a DNA profile that establishes or confirms a cell line's genetic identity. When appropriate, we can provide testing using StemElite® to detect mouse feeder cell contamination.
 Mycoplasma Contaminant Detection
Authenticating cell cultures is a process that not only verifies the identity of the cell line, but also rules out any contaminants. Mycoplasma is one of the most prevalent contaminating agents in cell culture. Unlike most bacterial and fungal contaminants, which are easily detected by changes in visual appearance of the culture, Mycoplasma contamination cannot be detected without further analysis.
Mycoplasma are parasitic, unicellular organisms that have the ability to induce chromosomal aberrations and metabolic changes, as well as alter a cell line's growth pattern, in some cases leading to the death of the cell line. These changes can affect the experimental results derived from infected cell lines.
To rule out Mycoplasma in your human, mouse, or other cell cultures, DDC offers the most comprehensive DNA detection test that will screen for 8 different genus and 209 species, including the 19 most common contaminants found in cell cultures (indicated by ++ in the list below).
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A. laidlawii (++)
M. arginini (++)
M. arthritidis
M. bovis
M. cloacale
M. falconis
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M. faucium (++)
M. fermentans (++)
M. genitalium
M. hominis
M. hyorhinis (++)
M. hyosynoviae
M. opalescens
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M. orale (++)
M. pirum
M. pneumonia
M. salivarium
M. synoviae
U. urealyticum
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Ordering Information
Please note that if you are ordering both Human Cell Line Authentication and Mycoplasma Detection, you only need to provide one sample for both tests.
Call 1-888-362-3928 To Order!
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Human & Primary Cell Line Authentication |
Mycoplasma Contaminant Detection |
| Pricing |
Single Samples ........................... $149 each
50+ Samples (free shipping) ........ $119 each
Inquire about volume discounts for 10+ samples
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Single Samples ........................... $69* each
50+ Samples (free shipping) ......... $49 each
Inquire about volume discounts for 10+ samples
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| What We Need |
- Purified DNA - 10-20 μl at a minimum of 1 ng/μl
- Cell pellet containing 1x105 cells
- FTA card containing a maximum of 6 cell line samples
(Please spot cell suspension to fill circle on card)
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- Purified DNA - 10-20 μl at a minimum of 1 ng/μl
- Cell pellet containing 1x105 cells
- If you would like to use a FTA card please contact us
- Mycoplasma only: Cell culture supernatant 100μl
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| What You Receive |
- STR Allele Report
- Electropherogram
- Fast Turnaround Time - Results in 5 days
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- DNA Test Report indicating presence or absence of Mycoplasma.
- Report completed within 5 business days
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*When ordering Mycoplasma Contaminant Detection with Human Cell Line authentication, single samples are $49 each.
Ordering Instructions
- Download a PDF of the order form here or call DDC at 1-888-362-3928 to get started.
- Complete Order Form and fax to 1-800-363-1707
- Carefully follow shipping guidelines, and send samples to:
DNA Diagnostics Center
Attention: Cell Line ID
One DDC Way
Fairfield, OH 45014
- You will be notified upon sample arrival and receive a due date.
- Once the testing is completed, your results will be sent via your preferred method(s).
Sample Preparation and Shipping Guidelines
Please note the following sample preparation and shipping guidelines:
- If sending cells, prepare a cell pellet containing at least 1 x 105 cells.
1. Wash the centrifuged cell pellet with phosphate buffered saline (PBS)
at least three times and place the cell pellet in a 1-ml vial or tube.
2. Ship the cell pellet on blue ice packs or dry ice.
- If performing your own DNA isolation, please send 10 - 20 μl of purified DNA (at a minimum concentration of 1 ng/μl). We recommend using TE-4 buffer (10mM Tris-HCl, 0.1mM EDTA [pH 8.0]). Please provide the concentration of DNA, if known.
- DNA samples in solution should be shipped on blue ice packs or dry ice. Dried DNA samples can be shipped at room temperature.
- If you would like to use a FTA card please contact us at 1-888-362-3928.
- Fill out the shipment form and send the samples to our laboratory via overnight courier.
DNA Diagnostics Center
Attention: Cell Line ID
One DDC Way
Fairfield, OH 45014
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ONLINE RESULTS 
